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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
01/11/2021 |
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Actualizado : |
01/11/2021 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
FERREIRA, V.; GONZÁLEZ-ARCOS, M.; PIANZZOLA, M.J.; COLL, N.S.; SIRI, M.I.; VALLS, M. |
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Afiliación : |
VIRGINIA FERREIRA, Área Microbiología, Departamento de Biociencias (DEPBIO), Facultad de Química, Universidad de la República, Montevideo, Uruguay; MATIAS GONZÁLEZ-ARCOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JULIA PIANZZOLA, Área Microbiología, Departamento de Biociencias (DEPBIO), Facultad de Química, Universidad de la República, Montevideo, Uruguay; NÚRIA S. COLL, Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain; MARÍA INÉS SIRI, Área Microbiología, Departamento de Biociencias (DEPBIO), Facultad de Química, Universidad de la República, Montevideo, Uruguay; MARC VALLS, Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain; Department of Genetics, University of Barcelona, Bellaterra, Catalonia, Spain. |
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Título : |
Molecular detection of Ralstonia solanacearum to facilitate breeding for resistance to bacterial wilt in potato. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
In: Dobnik D., Gruden K., Ram?ak ?., Coll A. (eds). Solanum tuberosum. Methods in Molecular Biology, 2021, vol 2354. Humana, New York, NY. doi: https://doi.org/10.1007/978-1-0716-1609-3_18 |
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Serie : |
eBook Packages Springer Protocols, (Methods in Molecular Biology, volume 2354). |
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ISBN : |
978-1-0716-1608-6 (print) / 978-1-0716-1609-3 (e-book) |
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ISSN : |
1064-3745 (print) / 1940-6029 (electronic) |
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DOI : |
10.1007/978-1-0716-1609-3_18 |
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Idioma : |
Inglés |
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Notas : |
Article history: First Online 27 August 2021. |
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Contenido : |
ABSTRACT. - Potato bacterial wilt is caused by the devastating bacterial pathogen Ralstonia solanacearum. Quantitative resistance to this disease has been and is currently introgressed from a number of wild relatives into cultivated varieties through laborious breeding programs. Here, we present two methods that we have developed to facilitate the screening for resistance to bacterial wilt in potato. The first one uses R. solanacearum reporter strains constitutively expressing the luxCDABE operon or the green fluorescent protein (gfp) to follow pathogen colonization in potato germplasm. Luminescent strains are used for nondestructive live imaging, while fluorescent ones enable precise pathogen visualization inside the plant tissues through confocal microscopy. The second method is a BIO-multiplex-PCR assay that is useful for sensitive and specific detection of viable R. solanacearum (IIB-1) cells in latently infected potato plants. This BIO-multiplex-PCR assay can specifically detect IIB-1 sequevar strains as well as strains belonging to all four R. solanacearum phylotypes and is sensitive enough to detect without DNA extraction ten bacterial cells per mL in complex samples. The described methods allow the detection of latent infections in roots and stems of asymptomatic plants and were shown to be efficient tools to assist potato breeding programs. © 2021, Springer Science+Business Media, LLC, part of Springer Nature. |
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Palabras claves : |
Bacterial wilt; Disease resistance; Plant breeding; Potato brown rot; Ralstonia solanacearum; Solanum tuberosum. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02607naa a2200301 a 4500
001 1062509
005 2021-11-01
008 2021 bl uuuu u00u1 u #d
022 $a1064-3745 (print) / 1940-6029 (electronic)
024 7 $a10.1007/978-1-0716-1609-3_18$2DOI
100 1 $aFERREIRA, V.
245 $aMolecular detection of Ralstonia solanacearum to facilitate breeding for resistance to bacterial wilt in potato.$h[electronic resource]
260 $c2021
490 $aeBook Packages Springer Protocols, (Methods in Molecular Biology, volume 2354).
500 $aArticle history: First Online 27 August 2021.
520 $aABSTRACT. - Potato bacterial wilt is caused by the devastating bacterial pathogen Ralstonia solanacearum. Quantitative resistance to this disease has been and is currently introgressed from a number of wild relatives into cultivated varieties through laborious breeding programs. Here, we present two methods that we have developed to facilitate the screening for resistance to bacterial wilt in potato. The first one uses R. solanacearum reporter strains constitutively expressing the luxCDABE operon or the green fluorescent protein (gfp) to follow pathogen colonization in potato germplasm. Luminescent strains are used for nondestructive live imaging, while fluorescent ones enable precise pathogen visualization inside the plant tissues through confocal microscopy. The second method is a BIO-multiplex-PCR assay that is useful for sensitive and specific detection of viable R. solanacearum (IIB-1) cells in latently infected potato plants. This BIO-multiplex-PCR assay can specifically detect IIB-1 sequevar strains as well as strains belonging to all four R. solanacearum phylotypes and is sensitive enough to detect without DNA extraction ten bacterial cells per mL in complex samples. The described methods allow the detection of latent infections in roots and stems of asymptomatic plants and were shown to be efficient tools to assist potato breeding programs. © 2021, Springer Science+Business Media, LLC, part of Springer Nature.
653 $aBacterial wilt
653 $aDisease resistance
653 $aPlant breeding
653 $aPotato brown rot
653 $aRalstonia solanacearum
653 $aSolanum tuberosum
700 1 $aGONZÁLEZ-ARCOS, M.
700 1 $aPIANZZOLA, M.J.
700 1 $aCOLL, N.S.
700 1 $aSIRI, M.I.
700 1 $aVALLS, M.
773 $tIn: Dobnik D., Gruden K., Ram?ak ?., Coll A. (eds). Solanum tuberosum. Methods in Molecular Biology, 2021, vol 2354. Humana, New York, NY. doi: https://doi.org/10.1007/978-1-0716-1609-3_18
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
18/11/2015 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
BLUMETTO, O.; RUGGIA, A.; DALMAU, A.; ESTELLÉS, F.; VILLAGRÁ, A. |
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Afiliación : |
OSCAR RICARDO BLUMETTO VELAZCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDREA PAOLA RUGGIA CHIESA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; A. DALMAU, IRTA (Institut de Recerca i Tecnologia Agroalimentáries); F. ESTELLÉS, Universidad Politécnica de Valencia (UPV); A. VILLAGRÁ, IVIA (Instituto Valenciano de Investigaciones Agrarias). |
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Título : |
Behavioural characterisation of Holstein steers in three different production systems. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Animal Production Science, 2015, volume 56, Issue 10, pages 1683-1692. |
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DOI : |
10.1071/AN15078 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 25 January 2014 / Accepted 30 April 2015 / Published online 26 August 2015. |
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Contenido : |
ABSTRACT.
The objective of the present study was to characterise the behaviour of Holstein steers in three different production systems. Forty-eight castrated Holstein males were randomly divided into three groups and allocated to the following three outdoor treatments: (T1) animals confined in a yard with an area of 210 m2, (T2) animals confined in a similar-sized yard but with 6 h of access to a pasture plot, (T3) animals maintained throughout the experiment on a pasture plot. Behaviour was recorded by scan sampling, 12 h a day (from 0700 hours to 1900 hours), 3 days per week, for 4 weeks evenly distributed from Week 7 to Week 16 of the experiment. So as to assess their patterns of behaviour, a negative binomial regression, correspondence analysis and logistic regressions were performed. Grazing was the predominant behaviour among Groups T2 and T3, while ?eating hay? was the most frequent behaviour among Group T1. For all treatments, lying was the second-most frequent behaviour. Despite animals in T2 having access to pasture for only half of the time with respect to those in T3, there was no difference between both treatments in the time spent grazing. Correspondence analysis of behaviour as a function of weather conditions showed that several behaviours were close to certain weather conditions, e.g. ?standing? and ?ruminating while standing? were closer to light rainy weather, while ?lying? or ?ruminating while lying? were more related to sunny weather.?Lying? tended to increase along the day in all treatments, while ?eating hay? increased along the day within Group T1, but decreased within Groups T2 and T3. It is concluded that the management conditions associated with the systems that were studied produced different behavioural patterns in the steers. Grazing behaviour is important for the animals, and the permanent or restricted possibility to perform it, determined by the production system, meant that the patterns of other behaviours changed to give priority to pasture intake. MenosABSTRACT.
The objective of the present study was to characterise the behaviour of Holstein steers in three different production systems. Forty-eight castrated Holstein males were randomly divided into three groups and allocated to the following three outdoor treatments: (T1) animals confined in a yard with an area of 210 m2, (T2) animals confined in a similar-sized yard but with 6 h of access to a pasture plot, (T3) animals maintained throughout the experiment on a pasture plot. Behaviour was recorded by scan sampling, 12 h a day (from 0700 hours to 1900 hours), 3 days per week, for 4 weeks evenly distributed from Week 7 to Week 16 of the experiment. So as to assess their patterns of behaviour, a negative binomial regression, correspondence analysis and logistic regressions were performed. Grazing was the predominant behaviour among Groups T2 and T3, while ?eating hay? was the most frequent behaviour among Group T1. For all treatments, lying was the second-most frequent behaviour. Despite animals in T2 having access to pasture for only half of the time with respect to those in T3, there was no difference between both treatments in the time spent grazing. Correspondence analysis of behaviour as a function of weather conditions showed that several behaviours were close to certain weather conditions, e.g. ?standing? and ?ruminating while standing? were closer to light rainy weather, while ?lying? or ?ruminating while lying? were more related to sunny weather.?Lying? tended to ... Presentar Todo |
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Palabras claves : |
BEHAVIOUR; CATTLE; FREE-RANGE SYSTEMS; WELFARE. |
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Thesagro : |
COMPORTAMIENTO ANIMAL; GANADO BOVINO. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 02867naa a2200265 a 4500
001 1053934
005 2019-10-09
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1071/AN15078$2DOI
100 1 $aBLUMETTO, O.
245 $aBehavioural characterisation of Holstein steers in three different production systems.$h[electronic resource]
260 $c2015
500 $aArticle history: Received 25 January 2014 / Accepted 30 April 2015 / Published online 26 August 2015.
520 $aABSTRACT. The objective of the present study was to characterise the behaviour of Holstein steers in three different production systems. Forty-eight castrated Holstein males were randomly divided into three groups and allocated to the following three outdoor treatments: (T1) animals confined in a yard with an area of 210 m2, (T2) animals confined in a similar-sized yard but with 6 h of access to a pasture plot, (T3) animals maintained throughout the experiment on a pasture plot. Behaviour was recorded by scan sampling, 12 h a day (from 0700 hours to 1900 hours), 3 days per week, for 4 weeks evenly distributed from Week 7 to Week 16 of the experiment. So as to assess their patterns of behaviour, a negative binomial regression, correspondence analysis and logistic regressions were performed. Grazing was the predominant behaviour among Groups T2 and T3, while ?eating hay? was the most frequent behaviour among Group T1. For all treatments, lying was the second-most frequent behaviour. Despite animals in T2 having access to pasture for only half of the time with respect to those in T3, there was no difference between both treatments in the time spent grazing. Correspondence analysis of behaviour as a function of weather conditions showed that several behaviours were close to certain weather conditions, e.g. ?standing? and ?ruminating while standing? were closer to light rainy weather, while ?lying? or ?ruminating while lying? were more related to sunny weather.?Lying? tended to increase along the day in all treatments, while ?eating hay? increased along the day within Group T1, but decreased within Groups T2 and T3. It is concluded that the management conditions associated with the systems that were studied produced different behavioural patterns in the steers. Grazing behaviour is important for the animals, and the permanent or restricted possibility to perform it, determined by the production system, meant that the patterns of other behaviours changed to give priority to pasture intake.
650 $aCOMPORTAMIENTO ANIMAL
650 $aGANADO BOVINO
653 $aBEHAVIOUR
653 $aCATTLE
653 $aFREE-RANGE SYSTEMS
653 $aWELFARE
700 1 $aRUGGIA, A.
700 1 $aDALMAU, A.
700 1 $aESTELLÉS, F.
700 1 $aVILLAGRÁ, A.
773 $tAnimal Production Science, 2015, volume 56, Issue 10, pages 1683-1692.
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